In Situ Detection of DNA Damage: Methods and Protocols

Vladimir V. Didenko, 2002, Methods in Molecular Biology

Overview

This volume presents a comprehensive collection of methods for the in situ detection of DNA damage and apoptosis. It details major techniques, including those originally designed for labeling apoptotic cells that can be adapted for DNA damage analysis, and vice versa. The book features readily reproducible techniques for identifying single and double-stranded DNA breaks in tissue sections using various enzymes. It also introduces novel instrumental methods employing ultrasound for in vivo and in situ detection, alongside flow and laser-scanning cytometry applications.

Who it's for

• Researchers studying DNA damage and apoptosis.
• Scientists developing or applying molecular biology techniques.
• Biologists and medical professionals analyzing cellular damage.

Key features

• Language: English
• Binding: Hardcover
• Pages: 336
• Publisher: Springer
• Year: 2002
• ISBN: 9780896039520
• Series: Methods in Molecular Biology