Single Nucleotide Polymorphisms: Methods and Protocols

Kwok Pui-Yan, 2003

Overview

This book presents a comprehensive collection of reproducible techniques for single nucleotide polymorphism (SNP) discovery and genotyping. It covers cutting-edge protocols for mutation/SNP detection, including denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. The volume also details powerful and up-to-date methods for genotyping SNPs, such as molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Who it's for

• Researchers involved in genetic analysis.
• Scientists studying mutation detection.
• Individuals working with genotyping techniques.

Key features

• Language: English
• Format: Hardcover
• Publisher: Springer
• Year: 2003
• Pages: 288
• ISBN: 9780896039681